Abstract

Tomato Spotted Wilt orthotospovirus (TSWV) is a member of the Orthotospovirus genus. It has extensive host and causes serious disease in many crops. In May 2021, virus-like symptoms such as chlorosis, mottling, leaf yellowing and necrosis were observed on Polygonum multiflorum (hereinafter as P. multiflorum) leaves in Kunming, Yunnan Province, China. Spherical viral particles with a diameter of 80-120nm were observed by TEM.RT-PCR and DNA sequencing analysis were performed, and the results showed that the sequence of N gene for this virus shared 99.20% identity with TSWV isolate from Shandong isolate (acc. no. MN861982.1).The obtained 777-bp consensus sequence named TSWN-N was deposited in GenBank (acc. no. OP800910).Phylogenetic tree analyses suggested that TSWV obtained from P. multiflorum plant samples clustered together into a large clade from Korea. Koch’s postulate was performed, and the virus was re-amplied from the inoculated plants. This is the first report of TSWV on P. multiflorum in China and provides early warning of virus disease on medicinal materials.

Keywords:Tomato spotted wilt orthotospovirus; Polygonum multiflorum; Electron microscopy; RT-PCR