Abstract

Currently, there are several methods available for hemoglobin screening, such as the spectrophotometric determination of hemoglobin, the spinning microhematocrit determination, and the copper sulphate density method. This cross-sectional comparative (analytical) study was conducted in the Sudan University of Science and Technology faculty of Medical Laboratory Sciences. This study aimed to determine any significant variation in hemoglobin concentration (g/dl) between capillary and venous blood. The sample consists of 100 healthy Sudanese adult volunteers, 41 males (41%) and 59 females (59%), aged 18-24 years, who have been tested. Paired blood samples (EDTA venous & capillary) were collected from each individual and analyzed for hemoglobin concentration by manual technique (HiCN). The result shows there was a significant difference in Hb concentration in capillary blood (mean 13.2g/ dl) and venous blood (mean 12.8g/dl) (P<0.001). Hemoglobin concentration is significantly higher in capillary blood compared to venous blood, with a mean difference of +0.4g/dL (13.2±1.53g/dL vs. 12.8±1.41g/dL, P=0.000).

Keywords:Hemoglobin; Capillary; Venous blood; Analytical variations; Cyanmethemoglobin; Sudan; Adult health