Abstract

The present study aimed at the toxicity of biosynthesized Iron oxide nanoparticles using Casia fistula leaf extract, aggregation in gills and biochemical parameters of Platy Xiphophorus maculatus. Casia fistula leaf extract was used for the synthesis of iron oxide nanoparticles and described using UVVisible spectrophotometer, Scanning Electron Microscope, Energy Dispersive X-Ray Spectroscopy, Fourier Transform Infrared Spectroscopy and X-Ray Diffraction. Aggregation of biosynthesized iron oxide nanoparticles in the gills and biochemical parameters were estimated by exposing platy for 7 days. UV-VIS image of iron oxide nanoparticles was observed at 371.79nm. SEM image was observed at the wavelength range 5 and 10mm. EDX spectrum shows Fe and O elements and located between 0.5 to 6.5KeV. FT-IR spectrum was ascertained in the line of 4000-400cm-1. XRD results confirmed the crystal expression, and the moderate size is 20±24nm. Aggregation of iron oxide nanoparticles in gills increased and biochemical parameters such as protein, carbohydrate and lipids in muscle and gill of platy were gradually decreased with increased concentration of iron oxide nanoparticles. The present study concluded that the biosynthesized iron oxide nanoparticles using Casia fistula leaf extract affect the biochemical parameters of platy.

Keywords: Keywords: Toxicity; Casia fistula; Biosynthesis; Iron oxide nanoparticles; Aggregation; Biochemical; Platy fish