1Department of Medical Laboratories Sciences, Faculty of Medical Technology, University of Tripoli, Tripoli, Libya
2Department of Medical Microbiology and Immunology, Faculty of Medicine, Tripoli University, P.O. Box 13497, Tripoli, Libya
*Corresponding author: Ellabib MS, Department of Medical Microbiology and Immunology, Faculty of Medicine, Tripoli University, P.O. Box 13497, Tripoli, Libya
Submission: October 25, 2021Published: November 10, 2021
ISSN:2637-7764Volume6 Issue5
Background: Various oral health measures have been applied throughout the world. The most common
way is to use a toothbrush and toothpaste. However, the traditional way of using chewing sticks is found
in several parts of the world. Chewing sticks have a mechanical cleansing action similar to a toothbrush
in addition to their antimicrobial effect.
Objective: To evaluate the efficacy of Salvadora persica (Miswak) products on oral microbial flora
(bacteria and fungi) in comparison with toothbrushes and toothpaste.
Method: A cross-sectional study was conducted on 60 randomly selected participants, during the
period from March to August 2021. An oral rinses sample was collected at 2-time intervals: Samples
were collected before use and after one week of use from two groups (a group used Miswak and group
using ordinary toothpaste). The samples were immediately processed for microbiological phenotypic
conventional methods and in vitro susceptibility testing of the microbial isolates to antimicrobial.
Result: The organisms isolated from Miswak group, toothbrush and toothpaste group were identified.
Among the bacterial isolates, Streptococci mutans was the most isolates 16 (53.33%) from Miswak group,
and in 17 (56.67%) of toothbrush & toothpaste group, followed by Lactobacillus 12 (54%) in Miswak
group and 10 (33.33%) toothbrush & toothpaste group. Staphylococci aureus was found in Miswak group
2 6.67%) and 3 10%) toothbrush & toothpaste groups respectively. Candida isolates was manly C. albicans
11 (36.67%) from Miswak group, and in 12 (40%) of toothbrush & toothpaste group, the second isolate
was C. dubliniensis 2 (6.687%) Miswak group, and in 6 (20%) toothbrush & toothpaste followed by C.
glabrata 1(3.33%) from Miswak group.
Conclusion: Miswak has a significant reduction effect on both bacteria, candida immediately and after a
week of use.
Keywords: Oral hygiene; Oral cavity; Salvadora persica (Miswak)