Abstract

Microbial lignocellulolytic enzymes play important role in the early stage of energy generation. Microbial cells produces various types of lignocelluloytic enzymes. Among that screening and cocktail of enzymes take significant role in the hydrolysis of complex lignin molecule into the simple form. Some strain produces the simple reducing sugars which are used for various energy related product formation using appropriate microorganism of different fermentation conditions.
Thermophilic Streptomyces sp. MCMB 375 partially characterized for the production optimization parameter for activity of cellulase enzyme. Optimum temperature is 50 °C, pH 10 with incubation time of 22hrs. It produces multiple forms of the endo and exo cellulases. The enzyme having broad substrate specificity. Its optimum temperature and work at alkaline pH so this would be used in the process of the fermentation for specific product formation. The degradation products generated could be evaluated using microscopy studies and specific action of the enzyme using the substrate specificity will be needed for further studies. The mechanism of action of enzyme need to study on broad range of substrate for efficient process development. AI driven further optimization parameters could be further improve the Process.

Keywords:Lignocellulolytic enzyme; Cellulases; Thermophiles; AI tool software optimization