Abstract

DNA crown cells (artificial cells), in which the outside of the membrane is covered with DNA, can be readily synthesized in vitro using sphingosine (SPH)-DNA-adenosine mixtures. These DNA crown cells can proliferate within egg whites in vivo. A previous report on the synthesis of synthetic DNA (E. coli) crown cells showed that such cells could be cultivated for more than approximately 2 months through seven generations which qualify the cells as a strain. The present study examined whether a strain of synthetic DNA crown cells could be prepared using synthetic DNA (Human placenta) crown cells. The process of synthetic DNA crown cell preparation is described in conjunction with microscopic observations.

Keywords:Synthetic DNA crown cells; Cell strain; Sphingosine-DNA; Cell culture