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Abstract

Approaches in Poultry, Dairy & Veterinary Sciences

A Recombinant Lipl21 Protein Vaccine from Five Leptospira Interrogans Strains Against Pathogenic Leptospiral Infection in Vietnam

Submission: September 21, 2020;Published: October 23, 2020

DOI: 10.31031/APDV.2020.08.000676

ISSN : 2576-9162
Volume8 Issue1

Abstract

Leptospirosis is an emerging zoonotic disease with globally public health problem in both animals and humans. Currently, there is no effective vaccine to prevent most of the circulating serovars in Vietnam because of the diversity of among leptospiral serovars. In this study, a highly immunogenic epitopes from LipL21 were designed using an in-silico approach. The recombinant protein was constructed from conserved sequences of five Leptospira Interrogans isolated Vietnamese strains. Immunization of mice with multi-epitope protein resulted in the antibodies production, as evidenced by MAT. Recombinant proteins can also significantly reduce Leptospire severity in animal hosts. In conclusion, rLipL21 can act as a vaccine capable of effectively and safely against the infection of five strains of Leptospira Interrogans in Vietnam.

Keywords: rLipL21; Recombinant protein vaccine; Leptospira Interrogans; Vietnam

Abbreviations: OMPs: Outer Membrane Proteins; rLipL21: Recombinant LipL21 Protein; MAT: Microscopic Agglutination Test; LB: Luria-Bertani medium

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