Abstract

Muong Lay black pig is an indigenous breed of livestock and has recently been listed for conservation in Vietnam. The aim of this study was to investigate the phylogenetic relationship of the Muong Lay black pig with other pig breeds by sequencing its mitochondrial genome (mtDNA). The PCR products were sequenced by Applied Biosystems 3500 Genetic Analyzer. The complete mtDNA sequence was 16,742bp in length and includes two rRNA (12S and 16S), 22 tRNA, 13 mRNA genes, and one control region (D-loop). The phylogenetic analysis was performed by comparing both complete mtDNA and D-loop sequences of Asian and European pig breeds. The results of phylogeny reconstruction revealed that Muong Lay black pig belonged to an Asian clade and clustered with Bihu and Banmei pig from Yangtze River Region and Yellow River Region, while reporting distinctly different phylogenetic relationships with the European breeds.

Keywords:Mitochondrial DNA; Muong lay black pig; Pig breeds; Phylogenetic relationships; Sus scrofa