Abstract

In the present study, compared the effect of two cryoprotectant medium (GEYC buffer and sperm freezeTm Solution) on sperm motility, kinematics, survival, morphology, and DNA fragmentation in a population of normozoospermia is compared. Semen samples from 20 healthy donors were divided and frozen with either GEYC or sperm freezTm Solution. Semen characteristics were evaluated before freezing and after thawing. Percentage of motility, viability, plasma membrane integrity, and DFI decreased significantly compared to prefreeze values in both groups. Post-thaw motility following removal of the freezing media did not show difference in specimens that were frozen in GEYC buffer compared to those frozen in Sperm Freeze Solution™ (P < 0.05). Similarly, specimens cryopreserved in GEYC buffer had no differences in sperm motility, viability, PMI, and DFI compared to aliquots that were frozen in Sperm Freeze Solution™ (P < 0.05).

Keywords: Human sperm; Cryopreservation; Cryoprotectant; GEYC buffer; Sperm Freeze Solution™