Abstract

Poor bone health is related with various bone disease that predisposes to fractures, while sufficient supplementation with calcium and vitamin D suggested prevention of bone diseases. The present study aimed to find the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on in vitro bone health parameters using MG-63 cells such as alkaline phosphates enzyme (ALP) activity, collagen levels, and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Lauree Ann Duprey-Reed and samples were defined as the Bio field Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability range 76% to 139% with safe and nontoxic profile of test samples on MG-63 cell line. ALP was significantly increased by171.8% and 189.7% at 10μg/mL in the UT-DMEM+BT-TI and BT-DMEM+BT-TI groups, respectively as compared with the untreated group.

In addition, 100% and 33.3% increased ALP at 0.1 and 10μg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. The level of collagen was significantly increased at 10μg/mL by 145.8% in the UT-DMEM+BT-TI group, while 65.8% and 145.8% at 1 and 10μg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 86.3% and 136.1% at 1 and 10μg/mL, respectively as compared with the untreated test item and DMEM group.

The percent of bone mineralization was significantly increased by 150%, 21%, and 73.2% at 0.1, 10, and 100μg/mL, respectively in the UTDMEM+ BT-TI group, while 258.3% and 126.8% at 0.1 and 100μg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 33.3%, 32.7%, and 187.4% at 0.1, 10, and 100μg/ mL, respectively as compared with the untreated group. Bio field Energy Treatment might be vital in promotion and maintenance of strong and healthy bones and quality of life by assisting them in maintaining optimal vitamin D levels. It regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer, and many autoimmune disorders.

Keywords:Bio field energy; Bone strength; Osteosarcoma cells; Vitamin D; Bone mineralization

Abbreviations: CAM: Complementary and Alternative Medicine; NCCAM: National Center for Complementary and Alternative Medicine; MG-63: Human Bone Osteosarcoma Cells; ALP: Alkaline Phosphatase; DMEM: Dulbecco’s Modified Eagle’s Medium; FBS: Fetal Bovine Serum; FBS: Fetal Bovine Serum; EDTA: Ethylene Diamine Tetra Acetic Acid; UT: Untreated, BT: Biofield Energy Treated, TI: Test Item