Abstract

Centella asiatica (L.) is commonly ingested and turned into a variety of products because it is rich in antioxidants and has a wide range of medicinal properties. The asiaticoside substance was used to compare various active ingredient-rich Asiatic pennywort clones. In a greenhouse with 50% shading, five rayong lines, a commercial nakhon pathom line, and regional lines grown in the provinces of Phrae, Phichit, and Chainat were propagated. Weed control and consistent watering three times per week were maintained, and the plants were ready for harvesting 90 days after planting. The observed plant was divided into three groups based on DNA analysis
a) Group 1 included Rayong and Nakhon Pathom lines,
b) Group 2 included Phichit and Nonthaburi lines, and
c) Group 3 included Phrae lines.

Vegetative growth parameters were discovered to differ depending on cultivation site and plant line. The Phrae trials produced fresh yields of 725-754kg/rai (1 rai=0.16 hectare) and dried weights of 73-81kg/ rai with no significant difference, whereas Rayong line gave the highest asiaticoside level of 44 %w/w and the local phrae line performed the lowest level of 0.27%w/w. The Phichit trial delivered a fresh yield of 805-1,012kg/rai and a dried weight of 70-86kg/rai, with the lowest asiaticoside content ranging from 0.1-0.12% w/w. The experiment in Chainat produced the highest fresh yields of 1,203-1,433kg/rai and dried weights of 179-315kg/rai, with the Nakhon Prathom line outweighing the other two lines in dried powder weight. According to all available information, the growing location of Asiatic pennywort may influence the asiaticoside production. This study, however, only included data from the cool growing season; additional trials in other seasons will be required to identify the best Asiatic pennywort plant line/lines with desirable asiaticoside content that are suitable for all cultivation climates.

Keywords:Centella asiatica; Vegetative growth; Asiaticoside