Abstract

The present research was carried out the evaluation of the anti-inflammatory property of methanolic extract of Cynodon dactylon and Acyclovir as individual and in combination by two different methods viz., Albumin denaturation assay and Membrane stabilization assay. Ten different concentration of Cynodon dactylon methanolic extract and Acyclovir solution (100, 200, 300, 400,500, 600, 700, 800, 900, 1000mg) were using in these studies. Antiinflammatory action was observed in dose dependent manner. In protein denaturation method at concentration of 1000ppm extract showed maximum protection (82.46%) and standard drug provided (73.23%) protection where as concentration of 500ppm acyclovir showed maximum protection (28.94%). The combination of both i.eat 1000ppm of extract and 500ppm of acyclovir showed maximum protection at the ratio of 80:20 (84.76%). Similarly, in membrane stabilization test, the selected extract at concentration of 1000ppm showed maximum membrane stabilization (89.1%) and standard drug provided (78.44%) protection, acyclovir showed maximum protection at concentration of 500ppm (28.62%).The combination of both i.e. at 1000ppm of extract and 500ppm of acyclovir showed maximum protection at the ratio of 80:20 (91.49%).Hence, from these results, we concluded that methanolic extract of Cynodon dactylon methanolic extract and acyclovir shows synergistic effect at different concentration when compared to standard NSAID drug (Diclofenac sodium). In addition, phytochemical analysis of Cynodon dactylon methanolic extract showed the presence of alkaloids, carbohydrates, flavonoids, glycosides, proteins, phytosterol etc. It reveals that these phytochemical constituents are responsible to maximum protection of protein denaturation and albumin denaturation and membrane stabilization assay.

Keywords: Cynodon dactylon extract; Acyclovir; Albumin denaturation; Membrane stabilization; Phytochemical analysis