Abstract

Synthesized DNA crown cells (artificial cells), which can proliferate within egg white in vivo, can be prepared in vitro using sphingosine (Sph)-DNA-adenosine-monolaurin compounds. Synthesized DNA crown cells form assemblies and proliferate in the presence of monolaurin in agar cultures. In previous experiments on synthetic DNA (Streptomyces) crown cells, the proliferation of cell-ring-like objects was observed on agar plates. Moreover, it was reported that antibiotics were produced in co-cultures of yeast and DNA (Streptomyces) crown cells, and that when the cells were used for beer-making, antibioticproducing cells were isolated from the cultures stimulated with egg white or monolaurin, suggesting that egg white and monolaurin stimulated the production of antibiotics in these cells. In the present experiments, I examined whether the antibiotic-producing cells could be isolated from the objects that form in the cultures of synthetic DNA (Streptomyces) crown cells stimulated with egg white and monolaurin. I successfully isolated the antibiotic-producing cells, which I named “antibiotic crown-Strept cells-a”, from the objects that formed in the cultures.

Keywords:Synthetic DNA (Streptomyces) crown cells; Agar plate cultures; Sphingosine-DNA; Antibiotic crown-strep cells; Monolaurin