Abstract

Preparing programs for the ecological balance of the environment and various fields of nature use is considered a priority issue in our country as well as in the whole world. One of the reasons that have a negative impact on the environment is the disposal of waste from poultry farms in various ways. Taking into account this problem, we have developed a method of extracting hyaluronic acid, which is important for medicine and cosmetology, from the combs of roosters, which are discarded as waste after meat processing. In the scientific research laboratory of the Department of Pharmaceutical Technology and Management of the Azerbaijan Medical University, optimal technological parameters were developed to release hyaluronic acid in the maximum amount from rooster combs. As a result of the experiment, it was found that the degree of fineness of the raw material is 1mm, the number of extractions is 2 times, the extractant is 2% NaHCO₃, extraction conditions - 80 °C, extraction time - 30 minutes, precipitation agent - 2% citric acid, yield percentage is 4.87-4.89%. We prepared a 1% solution of extracted HA based on optimal parameters, and some quality criteria were evaluated at the Analytical Expertise Center of the Ministry of Health of the Republic of Azerbaijan. It was determined that the pH of the solution is 7.65. The presence of Hyaluronic acid was determined by spectrophotometric method (280nm max. absorption in UV-light, optical density 0.441). The solubility of hyaluronic acid was tested and found that HA was gradually but completely dissolved in water, forming a viscous, colorless, slightly opalescent gel. Taking into account the protection of the environment from waste, as a scientific researcher, I believe that the reprocessing of slaughtered rooster heads in poultry enterprises will, on the one hand, promote the prevention of environmental pollution and, on the other hand, the implementation of the production process of hyaluronic acid, which has a positive effect on human health.

Keywords:Environmental protection; Rooster comb; Optimal technology parameters; Hyaluronic acid; Extraction