Abstract

In vitro propagation is one of the essential propagation methods and growth of rose (Rosa miniature). In this study, we used young, healthy shoots harboring one or two axillary buds of miniature rose as explants and MS medium as a basal medium containing several concentrations (0,0.5,1,2, and 4mg/L) of 6-Benzyl adenine (BA) and Indole-3-butyric acid (IBA) as growth regulators on the shoot and root regeneration. Our results illustrated that the highest number and most extended shoots were observed on MS medium containing 1mg/L BA. In MS medium with 0 and 0.5mg/L BA, the main shoot just could be grown without any multiplicities. BA had a positive effect on the enhancement of shoot induction. For rooting, a maximum number of rooted shoots were obtained on MS medium supplemented with 1mg/L IBA. Our observation showed the indispensable role of IBA during the rooting stage. In conclusion, we have established a tissue culture system by which micro propagated rosa miniature was rooted in vitro successfully.

Keywords: Benzyladenine; Indolebutyric acid; Micropropagation; Miniature rose