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Abstract
Significances of Bioengineering & Biosciences
Influence of Conditions of GC- Flame Ionization Detector Analysis on Parameters of Peaks in Highly Concentrated Compounds
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Open or CloseLudmila Yarmolinsky1, Leonid Yarmolinsky2, Khalfin B1,3, Budovsky A4 and
Ben-Shabat S3*
1Eastern R&D Center, Israel
2Arnie Miller Laboratories, Israel
3Faculty of Health Sciences, Ben-Gurion University of the Negev, Israel
4Research & Development Authority, Barzilai University Medical Center, Israel
*Corresponding author:Shimon Ben- Shabat, Faculty of Health Sciences, Ben- Gurion University of the Negev, Beer- Sheva, Israel
Submission: August 02, 2021; Published: August 31, 2021
ISSN 2637-8078Volume 5 Issue 2
Abstract
The aim of this research was to investigate an influence of flow velocity on the main parameters (retention time, tailing factor, relative area and number of theoretical plates) of peaks belonging to volatile compounds in the GC- Flame ionization detector analysis. Our results showed that as the flow increases, the retention time declines significantly (p<0.01). The other above-mentioned parameters of peaks also change depending on the characteristics of the solvents. The obtained results provide a platform for increasing accuracy of impurity tests for many volatile compounds and development of novel methods of analysis.
Keywords: GC-FID; Flow velocity; Tailing factor; Theoretical plate
Abbreviations: FID: Flame Ionization Detector
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