1Florida A&M University, USA
2Department of Civil & Environmental Engineering, FAMU-FSU College of Engineering, USA
3University of Georgia, Aiken, USA
*Corresponding author: Victor Ibeanusi, School of the Environment, 1515 S Martin Luther King Jr Blvd, Suite 305B, FSH Science Research Center, Florida A & M University, Tallahassee, 32307, Florida, USA
Ashish Pathak, School of the Environment, Florida Agricultural and Mechanical University, USA
Submission: August 01, 2018; Published: August 07, 2018
ISSN 2637-8078Volume2 Issue3
A patented microbial systems process was conducted on microbial-mediated biodegradative mechanisms at a coal-pile run off basin discharged from the coal fired plant at Savannah River Site, Aiken, South Carolina (SC). One of the isolated strains, ATCC 55673, grew robustly on number of dissolved toxic metals. Genome-centric evaluation revealed the isolate to belong to the genus Bacillus with close affiliation to B. cereus, an aggressive polychlorinated biphenyl-degrader. At a coverage of 90x, the genome of strain ATCC 55673 consisted of 4,615,850 bases with a total number of 4,590 putative genes assembling into 51 contigs with an N50 contig length of 2,597,36 bases. Several gene homologues coding for resistance to heavy metals were identified, such as a suite of outer membrane efflux pump proteins like nickel/cobalt transporter regulators, peptide/nickel transport substrate and ATP-binding proteins, permease proteins, a high-affinity nickel-transport protein, and several genes for metabolism of aromatic compounds (Table 1).
Keywords: Biomineralization of uranium and heavy metals, organic waste; Metal resistance genes; Whole genome sequencing (WGS); Bacillus sp. (ATCC 55673)