Abstract

The study objective was to evaluate the cardioprotective activity of Biofield Energized test item (DMEM) in rat cardiomyocytes (H9c2) cells. The test item (DMEM medium) was divided into three parts, first part received one-time Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and was labeled as the one-time Biofield Energy Treated (BT-I) DMEM, while second part received the two-times Biofield Energy Treatment and is denoted as BT-II DMEM. The third part did not receive any treatment and defined as the untreated DMEM group. MTT cell viability assay of the test sample showed more than 95% viable cells in the BT-I and BT-II groups, respectively suggested a nontoxic nature of the test substance. The BT-II group showed 16.28% restoration of cytoprotective function compared to the t-BHP induced group. The level of lactate dehydrogenase (LDH) was significantly inhibited by 48.36% and 47.89% in the BT-I and BT-II groups, respectively compared to t-BHP induced group. Level of creatine kinasemyocardial band (CK-MB) was significantly suppressed by 59.5% in the BT-I and BT-II groups compared to the t-BHP induced group. Reactive oxygen species (ROS) level was significantly suppressed by 120.08% and 116.20% in the BT-I and BT-II groups, respectively. Besides, BT-I and BT-II groups showed 33% and 48.5% inhibition of apoptotic cells, respectively compared to the t-BHP induced group. Altogether, result suggested that Biofield Treated test items significantly improved different cardiac parameters. Thus, Consciousness Energy Healing (The Trivedi Effect®) Treatment could be utilized as a cardio-protectant against several cardiac disorders such as heart failure, coronary artery disease, heart attack, congenital heart disease, cardiomyopathy, arrhythmias, etc.

Keywords: The Trivedi Effect®; Consciousness energy healing; H9c2; Heart health; CK-MB; ROS; LDH; Apoptosis; Reactive oxygen species; Tert-Butyl hydroperoxide

Abbreviations: CAM: Complementary and Alternative Medicine; BT-I: One-Time Biofield Energy Treatment; BT-II: Two-Time Biofield Energy Treatment; DMEM: Dulbecco’s Modified Eagle Medium; FBS: Fetal Bovine Serum