Abstract

DNA crown cells (artificial cells), in which the outer membrane is covered with DNA, can be readily synthesized in vitro using Sphingosine (Sph)-DNA-adenosine mixtures. Previous experiments demonstrated that assemblies of synthetic DNA (E. coli) crown cells formed naturally or in response to the presence of inorganic substances, and that modified synthetic DNA crown cells were produced within such assemblies. Moreover, the cells in these assemblies formed crystal-like substances and the cells were regenerated with further monolaurin treatment. Recent reports examined whether DNA crown cells, including regenerated synthetic DNA (E. coli) crown cells, could be cultivated in vitro. The cultivation of synthetic DNA (E. coli) crown cells was performed using egg white as a culture medium and it was found that such cells exit in medium after culturing for 7 days.

The aim of this study was to obtain evidence on whether such DNA crown cells could be cultivated further. Cells including related objects that had been cultivated in primary cultures were transferred to new medium (egg white) and incubated for a further 7 days at 37 C. Then, their microscopic appearance of such cells and related objects were observed in medium. Here, it was demonstrated that cells including related objects exist in the medium after secondary culture and that synthetic DNA (E. coli) crown cells could be cultivated. Here, the microscopic appearance of these cells and related objects are shown.

Keywords:Synthetic DNA crown cells; Sphingosine-DNA; Cell proliferation; In vitro; Cultures