Abstract

The present study was aimed to evaluate the antioxidant and free-radical scavenging potential of the Biofield Energy Healing (The Trivedi Effect®) Treated novel proprietary test formulation in human liver cancer (HepG2) cells. The test formulation was divided into two parts. One part was denoted as the control, which did not receive the Biofield Energy Treatment, while the other part received the Biofield Treatment, and defined as the Biofield Energy Treated test formulation. The test formulation was evaluated for antioxidant enzymes activities such as superoxide dismutase (SOD), catalase (CAT), lipid peroxidation (LPO), cell viability against oxidative damage, and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) analysis. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay data showed that the test formulation was noticed as a safe and non-toxic profile. The experimental results showed that the SOD level was significantly increased by 26.57% and 45.55% at 0.0065 and 0.0033μg/ ml, respectively as compared with the untreated test formulation. Further, CAT enzyme activity was improved by 28.57% at 1.04μg/ml as compared with the untreated test formulation. Similarly, LPO activity was significantly decreased by 26.46%, 29.02%, and 32.16% at 10.41, 0.104, and 0.05μg/ml, respectively with respect to the untreated test formulation. Free-radical scavenging activity using ABTS assay was inhibited by 9.8% at 1% with respect to the untreated test formulation. Overall, data suggested that the Biofield Energy Treated test formulation has significantly improved the antioxidant functions and could be useful against many autoimmune and inflammatory diseases, stress management and prevention. It can also be used as anti-aging therapy and for the improvement of body’s detoxification process.

Keywords: Consciousness energy healing; The Trivedi effect®; Free-radical; Anti-oxidation; Oxidative stress; HepG2 cells