Abstract

Infectious diseases are responsible for millions of global deaths annually. The misuse and increasing failure of chemotherapy and antibiotic resistance exhibited by bacterial pathogens has prompted researchers for screening of new antimicrobials from prolific producers of novel natural products including marine sponges [Scypha]. In the present study, crude extracts of marine sponge in methanol was investigated for secondary metabolites (flavonoids, glycosides, phenols, saponins, tannins, terpenoids) and antibacterial effect of marine sponge was evaluated on (multidrug resistant MDR) strains of Bacillus subtilis (ATCC 6633), E. coli (ATCC 8739), Salmonella enterica (ATCC 14028), Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 27853) by agar well diffusion method. Ciprofloxacin was used as standard.

The methanolic extracts of Scypha tested positive for glycosides and terpenoids. Maximum zone of inhibition was observed with Bacillus subtilis which is 23±0mm and Staphylococcus aureus which is 20±0mm. The potency shown by these extracts recommends their use against multidrug resistant microorganisms. The present study suggests that the methanol extract of the marine sponge (Scypha) exhibited a potential antibacterial activity against the tested microorganisms and could be a potential source of new antimicrobial agents as well as secondary metabolites.

Keywords:Marine sponges; Antibacterial; Scypha; Multidrug resistant; Microorganism

Keywords:SD: Standard Deviation; MDR: Multidrug Resistant; ATCC: American Type Culture Collection; E: Extract 1; H: hours; C: Ciprofloxacin