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Abstract

Cohesive Journal of Microbiology & Infectious Disease

The Evaluation of the Speed-Oligo® Mycobacteria Assay for Identification of Mycobacterium spp. from Smear Positive and Negative Sputum Samples

  • Open or Close Gülnur T1*, İsmail C2, Hülya Ş3, Özgül K4 and Yusuf Ö5

    1 Department of Medical Microbiology, Adıyaman University, Turkey

    2 Atatürk Chest Diseases and Thoracic Surgery Training and Research Hospital, Turkey

    3 National Public Health Instıtute, Tuberculosis Reference and Research Laboratory, Turkey

    4 Department of Medical Microbiology, Gulhane Military Medical Academy, Turkey

    5 Department of Medical Biology, Adiyaman University, Turkey

    *Corresponding author: Gülnur Tarhan, Faculty of Medicine, Department of Medical Microbiology, Adıyaman University, Turkey

Submission: July 30, 2018; Published: September 24, 2018

DOI: 10.31031/CJMI.2018.02.000533

ISSN: 2578-0190
Volume2 Issue2

Abstract

Speed-oligo® Mycobacteria is an oligochromatographic test for the qualitative detection of Mycobacterium genus and the species of M. tuberculosis complex, directly in clinical samples. It is based on polymerase chain reaction targeting 16S rRNA and 16S-23S rRNA regions and double-reverse hybridization on a dipstick using probes bound to colloidal gold and to the membrane. In this study, we evaluated 25 type reference strains of mycobacteria (18 non-tuberculosis mycobacteria, M. tuberculosis H37Rv and M. tuberculosis H37Ra), 60 sputum samples (40 smear positive, 20 smear negative) collected from patient with suspected TB. All results were compared with microscopy, Löwenstein Jensen culture and Inno-Lipa (GenoType Mycobacterium CM/AS; Hain Lifescience, Germany). All smear positive sputum samples were positive with microscopy, culture and Speed-oligo® Mycobacteria. Of 20 smear negative sputum samples, 7 were culture positive. Of 7 culture positive samples, 3 were positive with microscopy and Speedoligo ® Mycobacteria. It is not effective to identify for M. intermedium, M. kansasii and M. xenopi.

Keywords: Tuberculosis; NTM; Speed oligo; PCR

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