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Abstract

Research in Pediatrics & Neonatology

Differentiation between C. glabrata and C. parapsilosis Isolated from Children with Diarrhea by PCR Amplification of the ITS1-5.8S-ITS2 rDNA

*Corresponding author:Sabah M Ali, Research Scholar, Department of Pediatrics, College of Medicine, Al-Al-Mustansiriyah University Baghdad , Iraq

Submission: February 27, 2018; Published: April 9, 2018

DOI: 10.31031/RPN.2018.02.000526

ISSN : 2576-9200
Volume2 Issue1

Abstract

Candida glabrata and Candida parapsilosis are most important non-albicans species which are considered pathogenic to human and isolated from children with diarrhea. The purpose of the study is the identification and differentiation between Candida glabrata and Candida parapsilosis among children with diarrhea by PCR and determination relation of this species with gender, age group and resident of children. Hundred stool samples were collected from children with diarrhea seeking health care at AL-Batool Hospital in Baqubah city. The Stool cultured on sabouraud dextrose agar, subcultured on Chromogenic agar and PCR methods identification. In the present study, the isolated were identified which 14/64 (21.9%) isolates were C. glabrata, while 11/64 (17.2%) isolate C. parapsilosis and 39/64 (60.9%) isolates were other Candida spp, the results showed there is a significant relationship between C. glabrata and C. parapsilosis with the gender of children. Candida glabrata and Candida parapsilosis have a close color between of them in Chromogenic agar but when PCR amplifying the ITS1-5.8S-ITS2rDNA region produced different size base pair that makes it easy differentiation between them.

Keywords: C. glabrata; C. parapsilosis; Diarrhea; ITS1-5.8S-ITS2rDNA; PCR

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