Abstract

The polymerase chain reaction (PCR) is enzymatic amplification of specific regions of DNA while the inverse polymerase chain reaction (IPCR) is used for obtaining flanking regions of unknown sequences without utilizing cloning techniques. The template for the reverse primers is a restriction fragment that has been ligated upon it to form a circle. The base changes and sequences of DNA can be amplified by using labeled substrates in polymerase chain reaction and non-denaturing polyacrylamide gels, the electrophoretic mobility of single-stranded nucleic acid depends not only on its size but also on its sequence. Mobility shifts have used to determine single base changes up to 200-base fragments. A comprehensive statistical analysis called χ2 test has done in order to evaluate the sample of 200 individuals of both experimental group and control group. The blood tests reports with detailed history was collected with the help of clinicians in NORI and entered in the Performa. The current study will determine various types of thyroid cancer with respect to genetic alterations in aforementioned populations.