Abstract

Cinnamomum zeylanicum have lot-of biological activities including antimicrobial, antioxidant and antifungal properties. Furthermore, cytotoxic and apoptotic activities of several constituents were identified throughout its biological properties. Bark of Cinnamomum zeylanicum (Lauraceae) collected respectively at Nanotechnology laboratory (ANGRAU, Tirupathi, India). In this study, microbiological aspects of scale formation in PVC pipelines bacteria and fungi were isolated. Stable Zn nanoparticles were formed by treating 90ml of 1mm zinc nitrate aqueous solution with 10ml of 10% bark extract. The formation of Cinnamomum zeylanicum bark extract mediated zinc nanoparticles (CZnNPs) was confirmed by UV-visible spectroscopic analysis and recorded the localized surface plasmon resonance (LSPR) at 270nm. Fourier transform infrared spectroscopic (FT-IR) analysis revealed that primary and secondary amine groups in combination with the proteins present in the bark extract are responsible for the reduction and stabilization of the CZnNPs. The morphology and crystalline phase of the nano crystals were determined by Transmission electron microscopy (TEM) and (XRD). The hydrodynamic diameter (20.3mm) and a positive zeta potential (10.4mV) were measured using the dynamic light scattering technique. The antimicrobial activity of CZnNPs was evaluated (in vitro) against fungi, Gram-negative and Gram-positive bacteria using disc diffusion method which were isolated from the scales formed in drinking water PVC pipelines.

Keywords:Zinc nanoparticles; Fungal sp; Gram negative; Gram positive bacteria; bark extract of cinnamomum zeylanicum