Abstract

Background: The SW982 cell line has been widely used as an in vitro model in functional and therapeutic studies of Synovial Sarcoma (SS). This cell line was established in 1974, and it is commercially available from the American Type Culture Collection (ATCC). Changes in the cytogenetic profile of the SW982 cell line since its initiation have not been well-established. This study cytogenetically characterized SW982 utilizing multiple techniques, including routine G-banded chromosome analysis, oligonucleotide array Comparative Genomic Hybridization (array CGH), and Fluorescence in Situ Hybridization (FISH).

Results: The SW982 cell line (20 metaphases) exhibited a polyploid karyotype, 90-91, XXXX, der(1)t(1;4)(q10;p10)×2,del(5)(q23q33),dup(7)(q31q35),der(9)t(9;13)(q10;q10)×2,der(9)t(4;9)(q10;p24)×2,der(18)t(18;22)(q23;q13),+20,del(22)(q11.2q12.3),+mar[cp20]. Compared to previous reports, this SW982 cell line exhibited chromosome structural rearrangements and copy number changes.

Conclusion: Accurately determining the genetic changes in the SW892 cell line since its initiation will provide guidance when making genotypic and phenotypic correlations, and for establishing authenticity when it is applied in biological studies.

Keywords: SW982; Synovial sarcoma; FISH; Array CGH; Chromosomal changes

Abbreviations: SS: Synovial Sarcoma; CGH: Comparative Genomic Hybridization; FISH: Fluorescence In Situ Hybridization; ATCC: American Type Culture Collection