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Modern Applications in Pharmacy & Pharmacology

Potential Application of Raman Micro-Spectroscopy as an In vitro Drug Screening and Companion Diagnostic Tool for Clinical Application: Chemotherapeutic Drug Mechanism of Action, Cellular Effects and Resistance

  • Open or Close Z Farhane1,2*, F Bonnier3 and H J Byrne1

    1FOCAS Research Institute, Dublin Institute of Technology, Ireland

    2School of Physics and clinical and optometric Sciences, Dublin Institute of Technology, Ireland

    3Université François Rabelais de Tours, France

    *Corresponding author: Z Farhane, FOCAS Research Institute, School of Physics and clinical & optometric Sciences, Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland

Submission: October 31, 2017; Published: November 13, 2017

DOI: 10.31031/MAPP.2017.01.000506

ISSN 2637-7756
Volume1 Issue2


To be considered as an in vitro companion diagnostics technique to screen for personalised therapies, Raman micro-spectroscopy should be able to monitor sub cellular interaction with chemotherapeutic drugs and to characterise cellular resistance. Investigations demonstrate the ability of Raman micro-spectroscopy not only to track the sub cellular accumulation of the drug as a function of time, but also to identify its mechanism of action, the subsequent cellular response and to identify cellular resistance. Despite the fact that different cell lines show different chemotherapeutic resistance, the chemical binding signature appears to be identical for anti-cancer drugs which belong to the same chemotherapeutic group, with implications of different mechanisms of action as a function of time and dose.

Keywords: Raman micro-spectroscopy; Chemotherapeutic drugs; Mechanism of action; Cellular effects; Resistance; Companion diagnostic

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