Abstract

DNA crown cells are artificial cells in which the outside of the membrane is covered with DNA. Such artificial cells are generated readily using sphingosine (SPH)-DNA-adenosine mixtures and can proliferate within egg whites. To date, many kinds of DNA crown cells have been synthesized using DNA from various donors.

During previous studies of DNA crown cells, it was observed that antibiotics are produced by the coculture of DNA crown cells with yeast (from beer). Based on these findings, I inferred that DNA crown cells may control the growth of yeast and that such DNA crown cells may multiply. In initial experiments to clarify such problems, Bacillus subtilis (natto), an organism that presents several advantages. were employed instead of yeast.

In the experiments described here, it was demonstrated that assemblies of many sizes and shapes are formed in the mixtures of DNA (Escherichia coli) crown cells synthesized using adenosine-monolaurin compounds with B. subtilis cells. Here, characteristic assemblies were observed to be generated from such mixtures. In some assemblies, objects resembling DNA crown cells and bacteria were observed, suggesting that such cells may be incorporated into these assemblies, with associated suppression of bacterial growth.

Keywords: DNA crown cells; Assembly; Bacillus subtilis; Sphingosine-DNA