Journal of Biotechnology & Bioresearch

Enhanced Succinic Acid Production in Escherichia coli by Model-Guided Metabolic Gene Knockout of pflA Using Glucose Carbon Source

  • Open or Close Bashir Sajo Mienda1*,Saidu Jibril2, Umar Ali3, Aminu Muhammad4, Rabiu Salihu1 and Aliyu Adamu5

    1Department of Microbiology & Biotechnology, Federal University Dutse, Nigeria

    2Department of Chemical Sciences, Federal University Kashere, Nigeria

    3Department of Chemistry, SuleLamido University Kafin Hausa, Nigeria

    4Department of Pure and Industrial Chemistry, Bayero University Kano, Nigeria

    5Department of Microbiology, Kaduna State University, Nigeria

    *Corresponding author: Bashir SajoMienda, Department of Microbiology & Biotechnology, Faculty of Science, Federal University Dutse, PMB 7156 Ibrahim Aliyu Bypass, Dutse, Jigawa State, Nigeria

Submission: May 30, 2018; Published: July 09, 2018


Succinic acid is an important platform and/or commodity or specialty chemical with a broad range of applications. The metabolic role of pyruvate formatelyase A (pflA) in relation to succinate production in Escherichia coli under anaerobic conditions from glucose substrate remained largely unspecified. Herein we identified pflAgene for the first time, as a novel gene knockout target for increasing succinate production in E. coli. Guided by E. coli reconstruction iJO1366, we engineered the E. coli host metabolism by deleting the pflA, thereby causing the up-regulation of glyceraldehyde- 3-phosphate dehydrogenase (GAPDH), which hypothetically increases the generation of NADH and the pool of phosphoenolpyruvate (PEP) in the central carbon metabolism, required for succinate production. This strategy produced succinic acid that is 4.78 fold (0.28g l-1 in 1day) from glucose substrate. This work elucidatesfor the first time that pflA is a novel gene deletion target for increasing succinic acid production in E. coli under anaerobic conditions. In addition, these results highlight the power of metabolic model in identifying novel gene deletion target and ultimately driving novel biological discovery.

Keywords: Escherichia coli genome-scale model;Metabolic gene knockout prediction;Pyruvate formatelyase (pflA); Glucose carbon source; Enhanced succinate production

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