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Abstract

Examines in Marine Biology & Oceanography

Transcriptome Analysis of Immune-Related Gene in Hybrid Yellow Catfish (Tachysurus Vachelli x Tachysurus Fulvidraco) in Reaction to Peptidoglycan Challenge

  • Open or CloseTingshuang Pan1,2*, Jun Ling1,2 and Ming Yan1,2

    1Fishery Institute of Anhui Academy of Agricultural Sciences, P.R. China

    2Key Laboratory of Aquaculture & Stock Enhancement in Anhui Province, P.R. China

    *Corresponding author:Tingshuang Pan, Fishery Institute of Anhui Academy of Agricultural Sciences, Key Laboratory of Aquaculture & Stock Enhancement in Anhui Province, Hefei, 230031, P.R. China

Submission: August 23, 2022;Published: December 01, 2022

DOI: 10.31031/EIMBO.2022.05.000607

ISSN : 2578-031X
Volume5 Issue2

Abstract

Hybrid yellow catfish (Tachysurus vachelli x Tachysurus fulvidraco) is one of the most important freshwater fish in China, intensive culturing has caused increased susceptibility to bacteria, viruses, and parasites. However, little is known about its adaptation mechanisms to pathogen infection. To better understand its immune system and immune related gene under pathogen infection, the transcriptome was analyzed by comparing the data of hybrid yellow catfish spleen stimulated by PGN and PBS. There were 285 Differentially Expressed Genes (DEGs) between PGN treated experimental groups and PBS control groups at 24h, including 213 up regulated and 72 down regulated DEGs. The expression of qRT-PCR from five up regulated DEGs and five down regulated DEGs were consistent with RNA-Seq. In the analysis of GO enrichment, DEGs in the spleens of PGN treated hybrid yellow catfish were significantly enriched in GO items, including biological process, cellular component, and molecular function items. The identified DEGs and enriched GO terms and KEGG pathways were useful for understanding the physical and biochemical performances, and the transcriptome data greatly enriched the genetic information of hybrid yellow catfish.

Keywords: Differentially expressed genes; Genes and genomes; Transcriptome; Ontology

Abbreviations:DEGs: Differentially Expressed Genes; PGN: Peptidoglycan; PBS: Phosphate Buffered Solution; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; LPS: Lipopolysaccharide; BP: Biological Process; CC: Cellular Component; MF: Molecular Function

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