Abstract

Background: The growing threat of bacterial resistance has spurred the exploration of herbal medicine as an alternative solution. Fenugreek seed, a remedy with a rich history spanning many decades, has effectively treated bacterial infections. The key to unlocking the potential of these traditional remedies lies in research into the bioactive substances found in plants. This research has revealed numerous therapeutic properties and validated their traditional applications, providing reassurance about the credibility of herbal medicine. Trigonella foenum-graecum L., one of the oldest medicinal herbs, is believed to be native to Asia or the Mediterranean region.
Objectives: This work aimed to determine the antimicrobial activity of Fenugreek (Trigonella foenum graecum) Extract on Bacteria Isolation from Different Clinical Specimen.
Methodology: It is a prospective cross-sectional study performed in Shendi City, Sudan, from December 2022 to January 2024, at the Microbiology lab, Faculty of Medical Laboratory Science. In this study,50 samples were collected from microbiology labs in Shendi City and identified using gram stain and biochemical tests. The susceptibility of gram-positive and gram-negative bacteria to the fenugreek was determined. We tested the fenugreek extract using the agar well diffusion method at 100%, 50%, and 25% w/v concentrations.
Result: Out of 50 clinical specimens, we identified 13 males and 37 females. Among the different clinical samples tested were thirty-five urine samples, ten wound swabs, two ear swabs, one blood, one sputum, and one aspirate. The clinical isolates were tested as follows: Escherichia coli 30%, Staphylococcus aureus 26%, Pseudomonas aeruginosa 14%, Serratia marcescens 10%, Providentia 6%, Citrobacter 4%, Proteus mirabilis 4%, Proteus vulgaris 2%, Klebsiella pneumoniae 2%, and Saprophytic 2%. Notably, all organisms showed 100% resistance to fenugreek. These results highlight the pressing need for further research using advanced extraction techniques.

Keywords:Antimicrobial; Fenugreek; Extract; Herbal medicine