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Abstract

Cohesive Journal of Microbiology & Infectious Disease

Bacterial Assessment of Stethoscopes Contamination with Multidrug- Resistant Bacteria

Submission: September 19, 2024;Published: October 30, 2024

DOI: 10.31031/CJMI.2024.07.000660

ISSN: 2578-0190
Volume7 Issue2

Abstract

Background: Healthcare environments are often home to organisms that can cause infections. One example is contaminated medical equipment, such as stethoscopes, which can harbor various nosocomial pathogens, including MDR bacteria. Hospital-acquired illnesses are acknowledged as severe issues in public health.
Objectives: To assess multi-drug-resistant bacteria isolated from stethoscopes in Shendi City, Sudan.
Methodology: This prospective cross-sectional study will take place in Shendi City at the microbiology laboratory of the faculty of medical laboratory sciences at Shendi University between March 2023 and January 2024. Using moistened sterile cotton swabs, 64 specimens were obtained from stethoscopes in various hospitals. The bacteria were then cultured in Macconkey and blood agar, and the isolated specimens were identified using biochemical assays, gram stain, and colonial morphology. Sensitivity testing was then carried out. A modified Kirby-Bauer disc diffusion method was used to determine which medications were resistant to bacteria.
Results: It was discovered that bacteria from 54 (84%) of the 64 cultured stethoscopes were contaminated. These bacteria included S. aureus 25 (46%), S. saprophyticus 2 (4%), S. epidermidis 7 (13%), B. cereus 13 (24%), Klebsiella pneumonia 3 (6%), and P. aeruginosa 4 (7%). be the most effective antibiotics for Gramnegative are amoxiclav (21%) and ceftriaxone (38%), and ciprofloxacin (45%) and Gentamicin (46%) for Gram-positive bacteria.
Conclusion: Significant bacterial contamination was found in the stethoscope. The isolates were resistant to several drug classes and might have been pathogens. It is essential to disinfect the stethoscope diaphragm both before and after every patient interaction.

Keywords:Contamination; Bacteria; Stethoscope; MDR; Disinfection; HIA

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