Abstract

Omphalitis is a non-contagious infection, which affects young poultry’s navel or yolk sac. It’s more likely to happen in an unclean environment, where opportunistic bacterial infection is more common. A study was conducted on the properties of bacteria associated with chicken omphalitis and their antibiotic profile. In this research, we took 55 samples from sick chickens on five separate farms in Dinajpur Sador; Satabganj; Fulbari; and Basherhat; and evaluated them using bacteriological; biochemical; molecular; and antibiotic sensitivity tests. Three bacteria genera (Escherichia coli; Staphylococci aureus; and Salmonella spp.) were isolated from yolk swab samples in omphalitis-infected chicks in this investigation. In both farms, E. coli consumed the maximum percentage of positive cases (94.11%; 80.00%; 83.33%; 71.42%; and 88.88%; respectively); Staphylococcus spp. came in second for the percentage of positive cases (58.82%; 50.00%; 66.66%; 57.14%; and 55.55% respectively); and the percentage of positive cases is the 3rd highest prevalence in Salmonella spp. (32.29 %; 40.00 %; 50.00 %; 42.65 %; and 33.33% respectively).

E. coli was the frequent bacteria (46%); subsequently Staphylococcus aureus (32%); and Salmonella spp. (22%). 576 bp DNA fragments were used to identify E. coli, confirming its identification. Isolates E. coli were sensitive to chloramphenicol; gentamycin; levofloxacin; azithromycin; and ciprofloxacin on the other hand resistant to erythromycin; amoxicillin; tetracycline; ampicillin; and Cefixime; according to the drug study. Salmonella spp. was resistant to erythromycin; azithromycin; ampicillin; and tetracycline but sensitive to gentamycin; Cefixime; chloramphenicol; levofloxacin; and ciprofloxacin. Chloramphenicol; levofloxacin; Cefixime; and ciprofloxacin were all effective against Staphylococcus aureus; however, gentamycin; tetracycline; ampicillin; erythromycin; and amoxicillin were not. The outcome of the antibiogram study; has found that all isolates were unaffected against most of the antibiotics; which is not respectable at all. To those antibiotics, which the isolates were susceptible; should be applied in recommended dose for treatment purposes and also need to maintain proper cleaning on environment

Keywords:Antibiotic sensitivity; Bacteria isolation rate; Chicken; Omphalitis; Yolk sac infection

Abbreviations:EMB: Eosine Methylene Blue; SS: Salmonella Shigella; NA: Nutrient Agar; BGA: Brilliant Green Agar; MSA: Manitol Salt Agar; MAC: MacConckey Agar; XLD: Xylose Deoxycholate Agar; TSI: Triple Sugar Iron