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1Faculty of Medicine, Departmenf of Medical Microbiology, Turkey
2Faculty of Medicine, Departmenf of Medical Microbiology, Turkey
3Faculty of Medicine, Departmenf of Medical Microbiology, Turkey
*Corresponding author:Gülnur Tarhan, Faculty of Medicine, Department of Medical Microbiology, Turkey
Submission: November 20, 2019; Published: July 17, 2020
ISSN: 2578-0190 Volume4 Issue1
Real time PCR is a widely used for molecular diagnosis of tuberculosis in routine diagnosis laboratory. There are many commercially avaliable system. The sensitivity of the system varies according to DNA extraction from the sample and target specific primers and probes and commercial systems. In this study, the sensitivity and specificity of AdvanSure TB/NTM real time PCR kit were evaluated for detecting mycobacteria in smear positive and negative sputum specimens of 35 active pulmonary tuberculosis patients. All specimens were corrected using EZN staining, solid (Löwenstein Jensen) and liquid culture (MGIT). Culture and clinical findings were used as gold standards in the evaluation of all PCR results. Overall true positivity was found 25 ( 71.42%). When smear negative samples were evaluated together with culture results, only 3 patients were found positive by PCR test in 10 samples (30%).
Keywords: Mycobacterium tuberculosis; Non-tuberculous mycobacterium; AdvanSure TB/NTM; realtime PCR
Ph.D in Agriculture from Faculty of Agriculture, Tohoku University
Research Professor, PhD, Holistic Research Institute
Professor, Chief Doctor, Director of Department of Pediatric Surgery, Associate Director of Department of Surgery, Doctoral Supervisor Tongji hospital, Tongji medical college, Huazhong University of Science and Technology
Senior Research Engineer and Professor, Center for Refining and Petrochemicals, Research Institute, King Fahd University of Petroleum and Minerals (KFUPM), Dhahran, Saudi Arabia
Fellow of International Agency for Standards and Ratings (IASR), Edith Cowan University, Sarich Neuroscience Research Institute
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