Abstract

Introduction: Laryngoscope is using for endotracheal intubations. Inadequate decontamination of laryngoscope can develop nosocomial infections. If the blade isn’t disconnecting from laryngoscope after intubation can be transmit infections to handle. This study considering prevalence and types of bacteria isolated separately from laryngoscope blades and handles.

Method: This cross-sectional study was in the operating rooms of the educational and treatment center of Qazvin University of Medical Science in province Qazvin in Iran at 2018. 40 Laryngoscope blades and 40 laryngoscope handles were sampled after disinfection, with 4 methods disinfection: (1-Water, Povidon Iodine 7.5%, Ethanol 70%), (2-Water, Povidon Iodine 7.5%, Deconex (guaifenesin and phenylephrine 53 plus), (3-Water, Povidon Iodine 7.5%) and (4-Ethanol 70%)”. Samples were cultured on Mueller Hinton 5% sheep blood agar plate, MacConkey agar and Manitol salt agar. Inoculating plates were incubated at 37 ᵒC for 48 hours. Dominant microorganism and other growth bacteria identified comparatively.

Results: Nine various types of microorganisms were isolated and determined that handles were more Contamination than blades. Most negative results derived from blades and handles that were separated from each other and also for blades that were kept on gauze and separated from other instruments.

Conclusion: Performance of a standard disinfection method by software spss22 for both part of laryngoscope (blade and handle) seems to be necessary. Considering a special spot (like a special dish) for laryngoscope separated from other instruments may prevent the development of nosocomial infection.

Keywords: Laryngoscope; Nosocomial infection; Intubation