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Abstract

Cohesive Journal of Microbiology & Infectious Disease

Human Monocyte/Macrophage Fungicidal Activity of GM-CSF Against Paracoccidioides brasiliensis Depends on ROS

Submission: January 17, 2018; Published: February 20, 2018

DOI: 10.31031/CJMI.2018.01.000511

ISSN: 2578-0190
Volume1 Issue3

Abstract

The ability of recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) to activate human monocytes/macrophages for virulent Paracoccidioides brasiliensis killing was evaluated. Peripheral blood monocytes (MO) and monocyte-derived macrophages (M∅) were activated with different concentrations of GM-CSF. Afterwards, cells were challenged with P. brasiliensis strain 18 (Pb18) and the fungicidal activity was evaluated, plating and counting the Colony Forming Units (CFU) after 10 days. GM-CSF activated MO and M∅ for P. brasiliensis killling in a concentration-dependent manner. There was an association between this fungicidal activity and the high levels of H2O2 release by the activated cells. Moreover, the killing effect was inhibited by Catalase (CAT), confirming the role of H2O2 in this process. On the other hand, L-Monomethyl-Arginine (L-NMMA) had no effect on fungicidal activity, showing that nitric oxide (NO) is not involved in killing by human cells against P. brasiliensis. Based on these data, the role of GM-CSFactivated human cells in the innate defense mechanisms against P. brasiliensis is discussed.

Keywords: Paracoccidioidomycosis; Paracoccidioides brasiliensis; Human monocytes; Human macrophages; GM-CSF; Fungicidal activity; H2O2; NO; ROS

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