1,2Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy, Slovakia
3Institute of Chemistry SAS, Slovakia
4Department of Epizootology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy, Slovakia
*Corresponding author: Viera Revajová, Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81Košice, Slovakia
Submission: August 24, 2022;Published: September 15, 2022
ISSN : 2576-9162Volume9 Issue2
Immune response of jejunum, ileum and cecum was followed after administration of yeast-derived ß-Dglucan as binder and low dose of T-2 toxin (145.84μg.kg–1) contaminated diet (2 weeks) in chickens. For that purpose, expression of MUC-2, IgA, pIgR genomes, jejunal IgA+ IEL (intraepithelial lymphocytes), and IgA+ LPL (lamina propria lymphocytes) were evaluated. A total of one day old chickens were randomly divided into four groups: control (C), β-D-glucan (G), T-2toxin (T) and β-D-glucan+T-2 toxin (GT). β-Dglucan in jejunum did not influence the activity of MUC-2 and IgA gene expression in combined GT group, but alone T-2 toxin increased their expression. β-D-glucan alone positively influenced intraepithelial IgA+ lymphocytes (p<0.05) in jejunum compared to T group demonstrating suppressed density, and ileal MUC- 2 (p<0.001) compared to C and T group. On the other hand, chickens of GT group showed increased pIgR expression (p<0.001) together with IgA+ IEL and IgA+ LPL density in jejunum. Challenge with T-2 toxin showed beneficial effect of ß-D-glucan on jejunal IgA production. Moreover, immunomodulatory effect of low doses of T-2 toxin on IgA generation was indicated by improving pIgR gene expression in intestine.
Keywords:T-2 toxin; β-D-glucan; Immunity; Small intestine; Chickens