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Abstract

Approaches in Poultry, Dairy & Veterinary Sciences

Reaction of Immune Cells to Campylobacter Jejuni in Chicken PBMC Treated by Different Probiotic Bacteria In Vitro

Submission: January 27, 2020;Published: February 07, 2020

DOI: 10.31031/APDV.2020.07.000665

ISSN : 2576-9162
Volume7 Issue3

Abstract

The immune response of Enterococcus faecium AL41 (EF AL41), Enterococcus faecium H31 (EF H31), and Lactobacillus fermentum AD1 (LF AD1) against Campylobacter jejuni CCM6191 (CJ) were investigated in vitro by flow cytometry and immunophenotyping of cultivated peripheral blood mononuclear cells (PBMC). It may be postulated that using of probiotic bacteria strains in single arrangement during 48h in vitro cultivation displayed different influence to evaluated T (CD3, CD4, CD8) and B (BU1, IgA, IgM) immune cells. E. faecium AL41 acted predominantly 24h pi with C. jejuni while L. fermentum AD1 effect was detected 48 h pi. Both probiotic strains also displayed distinct manner of T and B cells activation. E. faecium H3 showed the lowest effect to both, controls and C. jejuni

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