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Approaches in Poultry, Dairy & Veterinary Sciences

Isolation of Major Pathogens from Cattle with Subclinical Mastitis and Determination of Antibiotic Susceptibilities

Submission: March 23, 2018;Published: April 19, 2018

DOI: 10.31031/APDV.2018.03.000566

ISSN: 2576-9162
Volume3 Issue4


Bacterial factors of bovine mastitis have two groups according to origin and transmission routes. The primary contagious agents are S. aureus, Streptococcus sp. and E. coli. These factors are spread widely, especially with handling and milking units. The scope of this study was to determine the isolation and antibiotic susceptibility of S. aureus, Streptococcus sp. and E. coli, which are very important for milk production and frequent agents of subclinical mastitis. In our study, 138 samples of dairy cows were taken aseptically from Aydin province with subclinical mastitis case. In conclusion, 102 (18.5%) S. aureus, 21 (3.8%) Streptococcus sp., and 15 (2.7%) E. coli were isolated from the 552 milk samples examined. After PCR with specific primers in the genotypic identification, all isolates were confirmed about bacterial identification. S. aureus, Streptococcus sp., and E. coli isolates were susceptible to antibiotics of amoxicillin-clavulanic acid, oxytetracycline, cefoperazone and cephalexin-kanamycin.

Keywords: Subclinical mastitis; S. aureus, Streptococcus sp; E. Coli; PCR; Antibiogram

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