Abstract

The administration of autologous adipose-derived stromal cells (ADSC) and platelet-rich fibrin (PRF) extract by intra-venous injections was investigated as a treatment for canine intervertebral disc disease (IVDD). IVDD is a disease that can cause several symptoms in domestic dogs, ranging from pain to partial or complete paralysis. Five dogs suffered from IVDD that caused hind-legs paralysis with deep pain perception, were treated with autologous adipose-derived stromal cells, vascular fraction, and platelet-rich fibrin (PRF) extract. Autologous adipose-derived stromal stem cells (ADSC) were expected to ameliorate symptoms of IVDD due to its anti-inflammatory and cell regenerative action. Five sequential intravenous injections applied in one-week interval for five weeks improved condition of treated dogs judged by patients gait score and exercise pattern.

Keywords: Intervertebral disc disease; Adipose stromal stem cells; Platelet-rich fibrin; Stromal vascular fraction

Abbreviations: ADSC: Adipose Derived Stromal Cells; PRF: Platelet Rich Fibrin; IVDD: Intervertebral Disc Disease; MRI: Magnetic Resonance Imaging; CT: Computed Tomography; TGF-β: Transforming Growth Factor; VEGF: Vascular Endothelial Growth Factor; EGF: Epidermal Growth Factor; PDGF: Platelet Derivative Growth Factor