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Research in Pediatrics & Neonatology

Profiling of Microbial Contamination in Internal Atmosphere of Hospital Ward

*Corresponding author: Asawari Raut, Department of Clinical Pharmacy, Bharati Vidyapeeth University, Poona College of Pharmacy, Erandwane, Pune-411038, Maharashtra State, India

Submission: February 02, 2018; Published: May 11, 2018

ISSN : 2576-9200
Volume2 Issue2


Background: Indoor air is the greatest propagating source of pathogenic microbes which causes significant contamination in the indoor hospital environment, principally in terms of nosocomial infections. Hence, microbiological testing is necessary to assess air contamination in indoor air of hospital.

Objective: The present study was undertaken to assess air contamination in different wards of the hospital to obtain a causative relationship between air contamination and risk of developing infections through microbiological testing.

Method: Microbiological sampling was performed in indoor environment of different wards namely pediatric ward, maternity ward, labor room, pediatric intensive care unit (PICU) and neonatal intensive care unit (NICU) of Bharati Hospital, Pune. Settle plate method was selected wherein McConkey’s Agar plates were used for isolation of Gram-negative bacteria, one of the pathogenic groups. The petri plates were then exposed for an hour in different wards and incubated at 37ºC for 24 hours. After incubation, the total colony forming units (CFU) were counted.

Results: It was found that highest CFU was present in the labor room. As compared to other petri dishes, 18±3Gram-negative bacteria colonies were seen in the labor room petri dishes. Presence of such contamination in labor room may occur due to improper ventilation and improper sanitization. During each labor, large amount of blood as well as amniotic fluid along with other body fluids are being spilled in the room which plays a significant role in promoting the growth of microorganisms irrespective of whether the mother had any infection during the labor.

Conclusion: Microbiological air contamination testing depicted that the labor room was the most contaminated ward. Proper ventilation and sanitization in the hospital wards with regular quality control would overcome the probability of hospital nosocomial infections thereby promoting safety of mother and infant health.

Keywords: Nosocomial infections; Microbiological Testing; Labor Room; Gram-negative bacteria

Abbreviations: PICU: Pediatric Intensive Care Unit; NICU: Neonatal Intensive Care Unit; CFU: Colony Forming Units; HAIs: Hospital Acquired Infections; MDR: Multi-Drug Resistant; UTI: Urinary Tract Infection

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