Department of Applied Computer Science, University of Winnipeg, Canada
*Corresponding author: Yangjun Chen, Department of Applied Computer Science, University of Winnipeg, Canada
Submission: March 13, 2018; Published: May 11, 2018
ISSN 2578-0247Volume1 Issue4
The recent development of next-generation sequencing has changed the way we carry out the molecular biology and genomic studies. It has allowed us to sequence a DNA (Deoxyribonucleic acid) sequence at a significantly increased base coverage, as well as at a much faster rate . This facilitates building an excellent platform for a whole genome sequencing, and for a variety of sequencing-based analyses, including gene expressions, mapping DNA-protein interactions, whole-transcriptome sequencing, and RNA (Ribonucleic acid) splicing profiles. For example, the RNA-Seq protocol , in which processed mRNA is converted to cDNA and then sequenced, is enabling the identification of previously unknown genes and alternative splice variants. The whole-genome sequencing of tumour cells can uncover previously unidentified cancer-initiating mutations.