Casey Napper1, Carolyn Olver1, Katia Hucl1, Katelynn Foth1 and Gregory Hansen2*
1Saskatchewan Health Authority, Jim Pattison Children’s Hospital, Canada
2Division of Pediatric Critical Care, Jim Pattison Children’s Hospital, Canada
*Corresponding author: Gregory Hansen, Division of Pediatric Critical Care, Jim Pattison Children’s Hospital, Canada
Submission: May 18, 2021;Published: May 27, 2021
ISSN:2640-9208Volume6 Issue1
Introduction: Recommendations for children with severe altered states of consciousness does not include ketogenic nutrition. The purpose of this study was to evaluate short-term quality control outcomes for a ketogenic diet in this population, document challenges, and guide next steps.
Methods: We performed a 19-month descriptive cohort study that included children, <17 years of age, admitted to the pediatric intensive care unit. Short-term quality control outcomes included increase in beta-hydroxybutyrate, number of episodes of blood glucose levels below 4 mmol/L, development of acidosis (pH < 7.3), and clinical development of kidney stones.
Results: Thirteen patients with stroke, traumatic brain injury or hypoxic ischemic encephalopathy were evaluated. The average beta-hydroxybutyrate level was 1.7 mmol/L, one patient (8%) developed hypoglycemia, seven patients (54%) developed acidosis, and no kidney stones were documented. Initiation of enteral nutrition was delayed, and the average protein intake was 0.58 g/kg.
Conclusion: Modifications to our ketogenic protocol included changes to the ketogenic ratio, and earlier wean to optimize protein, and the goal of positive ketones from both serum and urine testing only.
Keywords: Acute brain injuries; Children; Enteral formulas; Ketogenic diet; Intensive care
Abbreviations: ASC: Altered States of Consciousness; BHB: Beta-Hydroxy Butyrate; GCS: Glasgow Coma Scale; HIE: Hypoxic Ischemic Encephalopathy; PICU: Pediatric Intensive Care Unit; REE: Resting Energy Expenditure; TBI: Traumatic Brain Injury
Severe brain injury is the leading cause of mortality and morbidity in children injured over one year of age [1]. In the Pediatric Intensive Care Unit (PICU), severe Altered States of Consciousness (ASC) are typically seen after Traumatic Brain Injury (TBI), stroke, or Hypoxic Ischemic Encephalopathy (HIE) secondary to drowning, cardiac arrest, or asphyxiation. To improve outcomes for these patients, optimizing nutritional support continues to gain momentum. Ketogenic diets as a therapeutic in severe ASC is a burgeoning field. In brain injury animal models, ketones have demonstrated significant neuroprotective effects through improved mitochondrial metabolic efficiency, decreased oxidative stress, preventing metabolic deficits, attenuating apoptosis, and reducing excitotoxicity [2-10]. In humans, their use has been suggested or trialed in a number of chronic neurologic conditions including Alzheimer’s disease, severe refractory epilepsy, intracranial neoplasms, Parkinson’s [11], and mitochondrial diseases [12]. Human data on severe ASC and ketones is limited. In adults, Robertson et al. [13] studied the effect of glucose administration after head injury and reported how it was the major cause of suppressed ketogenesis and may increase lactic acidosis. Ritter et al. [14] concluded that a carbohydrate-free-diet not only maintained stable blood sugars but reduced cerebral edema. In critically ill pediatric patients with refractory status epilepticus. A therapeutic ketogenic diet was safe and led to improved seizure outcomes [15]. At present, recommendations for nutritional support in children with severe ACS does not include a ketogenic diet. Therefore, the purpose of this study was to evaluate short-term quality control outcomes for a ketogenic diet in severe pediatric ACS, document challenges, and guide next steps.
PICU ketogenic diet development
At our hospital there is a well-established ketogenic diet program for children with epilepsy. Significant consultation with this service was done prior to the development of our pilot ketogenic protocol and throughout the study period. Balancing ketosis and protein provision became the two major discussion points. Ketosis via starvation was not consistent with the benefits of early enteral nutrition in critical illness, and consequently was not considered. Additionally, although 1.5-3g/kg protein is suggested in TBI [16] to prevent loss of lean body mass, support growth, and avoid negative nitrogen balance [17], protein optimization below 1.5g/kg was expected to achieve ketosis.
Initial ketogenic protocol
Ketogenic and PICU dietitians were involved in the care of
each patient upon initiation and throughout the patient’s PICU
stay. Nutrition assessment occurred upon admission to the PICU,
and upon confirmation of the ketogenic diet initiation. Ketogenic
therapy duration was fourteen days of enteral feeds. Thereafter,
patients were transitioned over two to three days to either a
typical enteral feeding formula or a typical oral diet that was age
appropriate. The ketogenic diet plan included initiation of enteral
feeds with a ready-to feed ketogenic tube feed formula (KetoVie
4:1 Vanilla, Cambrooke Therapeutics) within 24-48h of admission.
The formula was hypercaloric (1.4kcal/ml), which was diluted
half and half with water (0.7kcal/ml), thereby providing a more
isocaloric concentration and mitigating possible gastrointestinal
intolerance. The ready-to-feed formula provided a 4:1 ketogenic
ratio (i.e. 4 grams fat: 1 gram combined carbohydrate + protein), a
lower net carbohydrate per milliliter allowed for a higher protein
concentration than comparable ketogenic formulas, and contained
added medium chain triglycerides and carnitine which assisted in
achieving ketosis. As admission weight increased, protein provision
was less optimal, and volume of protein supplementation via a liquid
hydrolyzed protein fortifier (LiquiProtein, Abbott) was increased.
The amount of added protein was weight-determined and ordered
in a standard amount added to the ketogenic formula:water
mixture. The addition of protein fortifier to the 4:1 ratio diluted
formula decreased the ketogenic ratio to 3.4:1 in the lowest weight
group, and progressively lower in each subsequent weight group,
with the heaviest patients receiving the lowest ketogenic ratio of
2:1, in order to optimize protein intake.
Our goals were to meet but not exceed energy requirements,
and to titrate caloric provision based on measured Resting Energy
Expenditure (REE). In absence of indirect calorimetry in our
PICU, we used a ventilator-derived calculation as a surrogate for
measured REE [18]. This surrogate measure is more accurate in
patients >15kg. Study Design and Patient Selection We performed
a 19-month prospective descriptive cohort study including
children <17 years of age to the PICU with severe ASC. Inclusion
criteria reflected i) patients with a Glasgow Coma Scale (GCS) ≤ 8
secondary to TBI; or ii) patients with a GCS ≤ 8, hyperlactemia and
absent electroencephalographic (EEG) reactivity secondary to a
stroke; or iii) patients with a GCS ≤8, hyperlactemia and absent EEG
reactivity secondary to HIE; and iv) greater than 15kg in weight for
accurate ventilatory estimates on REE to be made. The exclusion
criterion removed all patients with metabolic conditions that a
ketogenic diet would exacerbate including pyruvate carboxylase
deficiency, porphyria or fat metabolism disorders [carnitine
deficiency (primary), carnitine palmitoyltransferase deficiency,
carnitine translocase deficiency, b-oxidation defects, medium-chain
acyl dehydrogenase deficiency, long-chain acyl dehydrogenase
deficiency, short-chain acyl dehydrogenase deficiency, longchain
3-hydroxyacyl-CoA deficiency, and medium-chain 3-hydroxyacyl-
CoA deficiency]. Ethical approval was provided by The University
of Saskatchewan Research Ethics Board (U of S Bio #763).
Monitoring
Blood glucose was monitored every 4 hours until stable, with a hypoglycemia protocol in place for measurements <2.5mmol/L. Serum Beta Hydroxy Butyrate (BHB) was measured daily and urine ketones twice daily to evaluate ketosis. Blood gases were monitored daily to evaluate incidence of metabolic acidosis. Quality control outcomes and variables collected the following short-term quality control outcomes were evaluated over a two-week period: i) increase in BHB; ii) number of episodes of blood glucose levels below 4mmol/L; iii) development of acidosis (pH < 7.3); and iv) clinical development of kidney stones. The following variables were collected during the two-week period: age, sex, primary neurologic injury, daily estimated and measured REE, weight at admission and per PICU protocol when stable, daily arterial blood gases, blood glucose, record of sedation and neuromuscular blockade, neuroimaging, urinalysis, and EEG interpretations.
Statistical methods
Descriptive statistics were utilized, including mean and standard deviation, median or proportions where appropriate.
Thirteen patients with stroke, traumatic brain injury or hypoxic ischemic encephalopathy are summarized in Table 1. Individualized and cohort data on nutrition and quality control outcomes are summarized in Table 2. The average beta-hydroxybutyrate level was 1.7mmol/L, one patient (8%) developed hypoglycemia, seven patients (54%) developed acidosis, and no kidney stones were documented. Initiation of enteral nutrition was delayed, and average protein intake was 0.58cg/kg.
Table 1:Patient demographics and clinical characteristics (n=13).
HIE: Hypoxic Ischemic Encephalopathy; TBI: Traumatic Brain Injury. amean (standard deviation).
Table 2:Nutrition and biochemical outcomes (n=13).
REE: Resting Energy Expenditure; BHB: serum Beta Hydroxybutyrate. amean (standard deviation); btarget urine ketones 2+ to 3+ (8-16mmol/L); cmedian.
Managing thirteen pediatric patients with severe ACS and
ketogenic nutrition presented numerous challenges. However, these
challenges allowed for informed revisions and the creation of our
current protocol (Supplement 1) that may be considered by other
tertiary centers. Our ketogenic protocol aimed for two seemingly
opposing nutrition goals: adequate protein provision for critical
illness and ketosis for the goal of neuroprotection. Although we
recognized that a high protein ketogenic diet would be problematic
with the conversion of glucogenic amino acids into glucose
through gluconeogenesis, our mean protein provision remained
poor at 0.58 g/kg/day. Adding LiquiProtein at the onset of enteral
feeds was also not optimal, as this reduced the ketogenic ratio
and mitigated ketosis. When utilizing ketogenic diets for seizure
control, typical targets are urine ketones of 2+ to 3+ (8-16mmol/L)
on Chemstrip 7, BHB between 3-6mmol/L, and/or the reduction
of seizures. In our initial protocol, biochemical targets were used
to determine if optimal ketosis was achieved. Since the efficacy
of ketosis could not be observed through the absence of seizures,
it was unclear when our patient was “ketotic enough” to achieve
neuroprotection. Currently there is no human data on what level of
measurable ketones provide neuroprotection in severe ACS and/
or if the effect is titratable. Since a healthy individual consuming
a typical diet would not have urine or blood ketones present, are
the presence of any ketones indicating a benefit? Initiating and
advancing early enteral nutrition is a well-documented struggle
in the PICU. Multiple factors contributed to our delay in adequate
enteral nutrition including surgical intervention and/or other
procedures, high dose vasoactive medications affecting splanchnic
perfusion, medical imaging, and intubation/extubation attempts.
Each of these scenarios often requires lengthy fasting periods
which make it difficult to initiate and advance enteral nutrition to
goal volumes and often contribute to the development of iatrogenic
malnutrition [19].
Our initial goal was to meet the calculated REE with diluted
formula to avoid potential overfeeding and possible gastrointestinal
intolerance associated with hypercaloric feeds. We also assumed
that enteral nutrition would be running at approximately 100% of
the Holiday-Segar total fluid intake. However, in practice, the volume
available for nutrition due to numerous intravenous infusions
resulted in underfeeding with diluted formula. Consequently, the
formula required several adjustments of calorie and protein ratios
and created an opportunity for increased confusion when being
prepared by nursing staff. The intention of having nursing staff
prepare formula was to facilitate initiation of the protocol after
regular hours or in absence of a dietitian. In reality, the ketogenic
protocol was not started until after a discussion with a dietitian
during interdisciplinary rounds. Our initial protocol also aimed to
provide a ketogenic diet enterally for two weeks. It was assumed
that the patients would be unable to eat orally for at least two weeks due to their severe brain injury. Some patients, however,
were extubated earlier, with the decision to allow for a progression
to a standard oral diet. The goal duration for the patient to remain
on the ketogenic diet was then shortened to seven to fourteen days
and clarification was made to ensure no oral ketogenic diets would
be utilized.
It is worth mentioning that during the time of study, our facility
did not have access to indirect calorimetry. We used a surrogate
calculation utilizing carbon dioxide output from our ventilator
to glean measured REE and aimed to personalize our energy
goals towards it. Calculated REE tends to overestimate measured
REE [20], and children with traumatic brain injury are often
hypometabolic secondary to the various therapeutic treatments
such as hypothermic cooling, and neuromuscular blockade [21].
Our data was consistent with these observations and suggested
wide discrepancies between calculated and measured REE.
Based on the challenges noted, our current protocol (Supplement 1) reflects targeted ketosis for neuroprotection in the first ten days post brain injury, with the understanding that protein provision will remain suboptimal for the duration of the ketogenic diet. To improve ketosis, patients are started on a 4:1 ketogenic ratio. We chose to change our formula mixture to 70:30 KetoVie 4:1 Vanilla: water to provide 1 kcal/ml, given there is often less volume available for enteral nutrition. If a more complex ketogenic formula with modular products is required, it will be prepared by food services to prevent potential errors. Second, to optimize protein as soon as possible, we now start weaning off the ketogenic diet on day 10 (rather than day 14) and continue weaning over 2-4 days by adding additional protein until goal protein for TBI management (1.5-3g/kg/day) is achieved. After this, carbohydrates are added and a standard enteral formula for age is transitioned. Finally, the goal ranges for urine and serum ketones (2-3+ and 3-6mmol/L respectively) have been removed from the order set; instead, the presence of ketones from both serum and urine testing will be considered a successful level of ketosis for this patient population.
Ketogenic Diet Initiation for Severe Brain Injury Traumatic or Hypoxic Brain Injury with GCS ≤ 8 and ≥15kg
KETO-TBI PROTOCOL INITIATION ORDERS: (Please transcribe the following into Physicians Orders):
1. No dextrose-containing IV fluids
2. No carbohydrate-containing medications (see reverse
for reference); Consult Pharmacy re: alternatives to CHOcontaining
meds.
3. Blood glucose monitoring q4h until stable on goal feeds
1. If GST <2.5mmol/L, notify physician to correct with appropriate
per kg dextrose load. Recheck GST in 10min and consider more
frequent glucose monitoring thereafter.
4. Bedside urine ketones BID (using Chem-strip 7 strips) (GOAL:
Presence of ketones).
5. Blood gas and Serum Ketones (“Beta Ketone”) q24h (GOAL:
Presence of ketones). (Beta Ketone requires 0.5ml blood
drawn in a dark green tube)
6. If MRI performed, please inform Dr. Greg Hansen, and ask for
“MRS for ketone bodies” with the MRI request.
7. Enteral feeds initiated within 24-48h of admission; consult
PICU RD and Ketogenic Diet RD.
8. Feeds initiated as follows based on patient weight. (KetoVie
Liquid formula to be ordered from Food & Nutrition) (Figure
1).
Figure 1.
a) Ongoing Management: PICU RD and Ketogenic Diet RD
will adjust recipe as needed to optimize calories, protein, and
ketogenic ratio based on serum and urine ketone levels.
b) Length of Therapy: Diet to continue via tube-feed for
MINIMUM 1-week post-injury, up to 2 weeks if possible. At the tenday
mark RD will start adding extra protein, which will decrease the
ketogenic ratio, and may reduce ketosis. Diet will be transitioned
to a standard formula at 14 days. NO ORAL KETO DIETS will be
started, if the patient is eating orally prior to 14 days discontinue
the Keto Diet and Protocol (feed standard diet).
1) Diet Order Entry via SCM: RN to order KetoVie Liquid required
in a 24h period through SCM. Please add approximately 20%
extra to account for priming/spillage.
2) NOTE: Ketovie is only making up 70% of the total volume.
3) New Order Entry → Diet & Supplements → Tube Feeding
Pediatric → Tube Feed Product: KetoVie Liquid Volume: _ml
for 24 hours.
4) Keto Bloodwork Order Entry via SCM: Search laboratory
orders for “Beta Ketone”.
Medication pearls for Ketogenic Diet:
1. Most suspensions will contain sugar.
2. Most IV formulations are keto friendly, as long as they are not
mixed with dextrose.
3. At the start of the admission orders, please indicate to
pharmacy that patient is on a ketogenic diet and to review
medications.
4. Most tablets have low amounts of carbohydrate making them
a great alternative. If they are water soluble, we can dissolve
and dose tablets (eg. Dissolve 100mcg tablet in 10mL to get
10mcg/mL solution, or we can round to nearest ¼ or ½ tablet
size and crush and administer).
Commonly ordered medications in PICU for traumatic brain injury
Table 3.
HIE: Hypoxic Ischemic Encephalopathy; TBI: Traumatic Brain Injury. amean (standard deviation).
© 2021 Gregory Hansen. This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and build upon your work non-commercially.