Abstract

Moulds growth and contaminated green coffee beans, produced mycotoxins as secondary metabolites such as Ochratoxin A. Ochratoxin A have been reported carcinogen in human health. The aim of this research was determined the occurrence of Ochratoxin A in green coffee beans using C18 SPE cartridges as alternative clean-up method. Samples were collected from Argo puro mountain areas, Jember Indonesia during the coffee year. Samples were prepared with extraction, clean-up, separation, and quantification. The parameter of OTA was determined by HPLC-FL Detector fitted with C18 HPLC column. The result shown 9 of 10 samples were detected OTA with the highest concentration was 0.4319ppm and the lowest concentration was 0.0146ppm.

Keywords: Jember green coffee beans; Ochratoxin A; C18 SPE Cartridges