1 Faculty of Agricultural Technology, Indonesia
2 Faculty of Life and Environmental Science, Japan
*Corresponding author: Harada H, Faculty of Life and Environmental Science, Japan
Submission: February 13, 2019;Published: February 22, 2019
ISSN: 2640-9208Volume3 Issue3
Moulds growth and contaminated green coffee beans, produced mycotoxins as secondary metabolites such as Ochratoxin A. Ochratoxin A have been reported carcinogen in human health. The aim of this research was determined the occurrence of Ochratoxin A in green coffee beans using C18 SPE cartridges as alternative clean-up method. Samples were collected from Argo puro mountain areas, Jember Indonesia during the coffee year. Samples were prepared with extraction, clean-up, separation, and quantification. The parameter of OTA was determined by HPLC-FL Detector fitted with C18 HPLC column. The result shown 9 of 10 samples were detected OTA with the highest concentration was 0.4319ppm and the lowest concentration was 0.0146ppm.
Keywords: Jember green coffee beans; Ochratoxin A; C18 SPE Cartridges