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Novel Techniques in Nutrition and Food Science

Presence of Ochratoxin in a Green Coffee Beans Processed Naturally by Smallholder Farmers in Jember

  • Open or CloseRosavani MA1 and Harada H2*

    1 Faculty of Agricultural Technology, Indonesia

    2 Faculty of Life and Environmental Science, Japan

    *Corresponding author: Harada H, Faculty of Life and Environmental Science, Japan

Submission: February 13, 2019;Published: February 22, 2019

DOI: 10.31031/NTNF.2019.02.000565

ISSN: 2640-9208
Volume3 Issue3


Moulds growth and contaminated green coffee beans, produced mycotoxins as secondary metabolites such as Ochratoxin A. Ochratoxin A have been reported carcinogen in human health. The aim of this research was determined the occurrence of Ochratoxin A in green coffee beans using C18 SPE cartridges as alternative clean-up method. Samples were collected from Argo puro mountain areas, Jember Indonesia during the coffee year. Samples were prepared with extraction, clean-up, separation, and quantification. The parameter of OTA was determined by HPLC-FL Detector fitted with C18 HPLC column. The result shown 9 of 10 samples were detected OTA with the highest concentration was 0.4319ppm and the lowest concentration was 0.0146ppm.

Keywords: Jember green coffee beans; Ochratoxin A; C18 SPE Cartridges

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