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Novel Research in Sciences

Applications Utilizing CRISPR/Cas9

Submission: February 08, 2023;Published: February 24, 2023

ISSN 2639-0612
Volume11 Issue3


Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Cas (CRISPR-associated) system is an adaptive immune system used by prokaryotes, which has been adapted for many laboratory applications. Here, we illustrate applications of CRISPR/Cas9 in 7 areas:
(i) Genome engineering
(ii) Edition of single-stranded RNA (ssRNA)
(iii) High throughput gene screening
(iv) Creating disease models
(v) Live labelling of chromosomal loci
(vi) Epigenome editing
(vii) Regulation of endogenous gene expression

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