1Department of Medical Oncology, Pamukkale University Hospital, Turkey
1Department of Medical Pathology, Pamukkale University Hospital, Turkey
*Corresponding author: Serkan Degirmencioglu, Pamukkale University Hospital Fahri Goksin Oncology Center 20100 Denizli, Turkey
Submission: February 10, 2018; Published: March 06, 2018
Aim: Osteopontin is a multifunctional extracellular matrix glycoprotein that plays role in cell adhesion, migration and invasion. Increased expression of osteopontin is related to poor prognosis in various tumors. This study aims to evaluate the role of osteopontin expression in cases of metastatic and non-metastatic melanomas.
Materials and methods: A total of 26 cases of melanoma, including 13 metastatic and 13 non-metastatic cases, diagnosed in the Department of Pathology at the Pamukkale University, were included in this study. An osteopontin antibody was applied immunohistochemically to the blocks of the cases, including the primary and metastatic tumors. The degree of osteopontin expression was expressed semi-quantitatively as none/mild/ intermediate/severe, and the results were compared using parameters, such as histopathological characteristics (location, depth, cellular type, number of mitoses, number of positive lymph nodes, number of total lymph nodes and presence of ulcer), age, gender, stage, survival rate and progression-free survival, and the analysis were performed using the SPSS 16.0 package program.
Findings: Osteopontin levels demonstrated a low expression in six out of eight cases without lymph node metastasis. Osteopontin levels were found to be low in four cases in which a nevus was detected on a melanoma ground. However, no statistical significance was found since the number of cases was insufficient. Progression-free survival was 16.13+-2.5 months (95% CI 11.1–21.09) in cases with a low osteopontin level and 41.2+-15.6 months (95% CI 10.5–71.8) in cases with a high osteopontin level. No statistical significance was detected regarding survival.
Result: Osteopontin expression in melanoma cases can be considered a significant marker of lymph node involvement and ulcer presence on the basis of the melanoma. This study should be replicated with a large case series to investigate survival rates.
Keywords: Osteopontin; Malignant melanoma; Prognosis
Abbreviation Ldh: Serum Lactate Dehydrogenase; Opn: Osteopontin; Sibling: Small Integrin Binding Ligand N-Linked; Os: Overall Survival; Pfs: Progression-Free Survival; Ihc: Immunohistochemical; Snp: Single Nucleotide Polymorphism; Ifn: Interferon