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Modern Applications in Pharmacy & Pharmacology

Mechanistic Biomarker Estimation in Kidney, Lungs, and Artery after Treatment with the Biofield Energy Healing Based Test Formulation in Vitamin D3 Deficiency Diet (VDD) Induced Animal Model

Mahendra Kumar Trivedi1, Alice Branton1, Dahryn Trivedi1 and Snehasis Jana2*

1Trivedi Global, Inc., USA

2Trivedi Science Research Laboratory Pvt. Ltd., India

*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Thane (W), Maharashtra, India

Submission: February 23, 2021;Published: April 15, 2021

DOI: 10.31031/MAPP.2019.02.000543

ISSN 2637-7756
Volume2 Issue4


This present study evaluated the impact of Consciousness Energy Healing/Blessing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats, fed with Vitamin D3 Deficiency Diet (VDD) for the estimation of mechanistic biomarkers such as endothelin-1 and nitric oxide (NO) in kidney, lungs, and artery tissues. A novel proprietary test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The novel test formulation was divided into two parts; one part was defined as untreated test formulation, while the other of test formulation and three group of animals received Biofield Energy Healing Treatment/Blessing by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. Kidney endothelin-1 level was significantly increased by 33.9%, 94.6% (p<0.001), 19.2%, and 14.6% in Biofield Energy Treated/Blessed test formulation group (G5), Biofield Energy Treatment per se (G6), 15 days pre-treatment of Biofield Energy Treated test formulation (G7), and untreated test formulation to the Biofield Energy Treated animals (G9) groups, respectively as compared with the untreated group (G4); while 66.9% (p<0.001) in the G6 group as compared to the G2 group. Similarly, the level of NO in kidney was significant increased by 10.6% and 10.4% in the G7 and G8 groups, respectively as compared with the G4. However, lungs endothelin-1 was reported to be increased by 62.7%, 18.8%, 26.9%, and 35.5% in the G6, G7, G8, and G9 groups, respectively as compared to the G2. Similarly, the lungs NO level was significantly increased by 18.4% and 54.3% in the G6 and G7 groups, respectively, as compared with the G4. The level of endothelin-1 in artery was increased by 13.6%, 16.7%, and 28.2% in G5, G6, and G7 groups, respectively as compared with the G2 group. Similarly, the level of No in artery was increased by 107.4%, 65.4%, 71.9%, 59.7%, and 37.1% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2 group. Altogether, the Biofield Treated test formulation and Biofield Energy Treatment per se significantly used as an effective approach to improve the functioning of endothelin-1 and NO in kidney, lungs and artery that can be used against many disorders such as Alzheimer’s disease, dementias, brain cancer, epilepsy and other seizure disorders, mental disorders, and Parkinson’s. Overall, the results showed the significant slowdown the disease progression and disease related all other complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease group.

Keywords: Biofield treatment; Mechanistic biomarkers; The trivedi effect®; Endothelin-1; Vitamin D3 deficiency diet; Calcitriol


A biomarker could be considered as a characteristic that that acts as an indicator and could be measured effectively regarding the normal or pathologic biological processes. Its use has also been evident as the response indicator to any therapy. In this regard, the mechanistic biomarker is used as a concept, which is based on the idea that the biomarker testing results can be used further to guide the clinical management of disease. The mechanistic biomarker is basically used in the clinical diagnosis of symptomatic disease such as, the presence of infectious diseases could be detected by analysing the antibodies directed against specific pathogens (e.g., HIV or hepatitis virus); diagnosis of certain cancers by detecting the specific genetic aberrations (including myelodysplastic syndrome and chronic myelocytic leukemia) [1]. Endothelin 1 (ET-1), also known as Preproendothelin-1 (PPET1), is a potent vasoconstrictor produced by vascular endothelial cells [2]. In animals, the vitamin D deficiency has been reported to result in the cardiovascular consequence i.e., hypertension. Some research studies on rats reported that the deficiency in Vitamin D Receptor (VDR) may develop hypertension in them. It could be associated with the activation of the renin-angiotensin-aldosterone system, as vitamin D acts as a negative regulator of renin synthesis. The levels of endothelin-1 were estimated in kidney, lung and artery [3]. Besides, nitric Oxide is involved in the nociceptive process as an important neurotransmitter, where it is mainly related with the development of central sensitization in the dorsal horn of the spinal cord. The studies reported that vitamin D could inhibit the synthesis of Nitric Oxide Synthase (iNOS) (responsible for producing nitric oxide), in macrophages that activates microglia and astrocytes at the protein as well as m-RNA levels [4,5]. Inhibition of iNOS can be a potential mechanism for reducing pain and neuronal damage after injury or in diseases such as Parkinson’s disease, ischemia, and Acquired Immune-Deficiency Syndrome (AIDS). In our explanatory model, there was reduced NO production in the vitamin D3 deficient rats that results over time in arterial stiffening and increased pulse pressure. It further leads to altered collagen and elastin content in the aorta by long-term increases in mechanical strain as well as to cardiac functional impairment by chronically increased afterload at older ages. Thus, the levels of NO were estimated in kidney, lung and artery [6].

Thus, some standard mechanistic biomarkers of healthy ageing correlating with the overall health are the current utility as surrogate endpoints of research. Various pre-clinical and clinical trials have been focused to develop a novel formulation that works to improve the overall health. However, there is no such novel herbal-based test formulation was designed that can improve the overall organ health using cell based standard assays. There is currently no universally accepted test formulation, which improve the organ health biomarkers. With this respect, the novel test formulation was designed on the basis of best scientific literature, which is the combination of different minerals (selenium, zinc, iron, calcium, copper, and magnesium), vitamins (cyanocobalamin, ascorbic acid, pyridoxine HCl, alpha tocopherol, and cholecalciferol), β-carotene, cannabidiol isolate, and panax ginseng extract. This formulation is designed for overall health against many pathological bone health conditions. Minerals and vitamins present in the test formulation provide significant physiological support [7-9]. In addition, panax ginseng is one of the best reported medicinal plants that improve mental, physical abilities, cognitive health, and is potent immunomodulator [10,11], while biological importance of cannabidiol as has been widely reported in many pharmacological actions [12,13]. The test formulation and the animals per se were treated with the Biofield Energy by Biofield Energy Healer and were studied for mechanistic parameters. 

In recent years, Biofield Energy Treatment was reported in several scientific reports and clinical trials for the useful effects in case of cervical cancer patients [14], massage therapy [15], and many more. Complementary and Alternative Medicine (CAM) therapies have been reported that Biofield Therapies (or Healing Modalities) as one of the best preferred models of treatment with several benefits to enhance physical, mental and emotional human wellness. National Centre of Complementary and Integrative Health (NCCIH) has been recognized and accepted Biofield Energy Healing as a CAM along with other therapies such as deep breathing, yoga, Tai Chi, Qi Gong, chiropractic/osteopathic manipulation, meditation, massage, special diets, homeopathy, progressive relaxation, guided imagery, acupressure, acupuncture, relaxation techniques, hypnotherapy, healing touch, movement therapy, pilates, Ayurvedic medicine, traditional Chinese herbs and medicines, naturopathy, essential oils, aromatherapy, Reiki, and cranial sacral therapy. Human Biofield Energy has subtle energy that has the capacity to work in an effective manner [16,17]. Biofield Energy Healing Treatment/Blessing (the Trivedi Effect®) results has been published in numerous peer-reviewed science journals with significant outcomes in many scientific fields on various models in the metal science [18,19], agriculture science [20], microbiology [21,22], biotechnology [23,24], and improved bioavailability of various compounds [25,26], skin health [27,28], nutraceuticals [29], cancer research [30], bone health [31-33], overall human health and wellness. The present study was designed to study the mechanistic biomarkers such endothelin-1 and nitric oxide in kidney, lungs, and artery using ELISA assay in male Sprague Dawley rats in presence of VDD diet and novel test formulation.

Materials and Methods

Chemicals and reagents

Pyridoxine hydrochloride (vitamin B6), calcitriol, zinc chloride, magnesium (II) gluconate, and β-carotene (retinol, provit A) were purchased from TCI, Japan. Copper chloride, cyanocobalamin (vitamin B12), calcium chloride, vitamin E (Alpha-Tocopherol), cholecalciferol (vitamin D3), iron (II) sulfate, and Sodium Carboxymethyl Cellulose (Na-CMC) were procured from Sigma-Aldrich, USA. Ascorbic acid (vitamin C) and sodium selenate were obtained from Alfa Aesar, India. Cannabidiol isolate and panax ginseng extract were obtained from Panacea Phytoextracts, India and Standard Hemp Company, USA, respectively. For the estimation of mechanistic biomarkers, specific ELISA kits were used such as for detection of endothelin-1 and nitric oxide, which were procured from CUSABIO, USA and MyBioSource, USA respectively.

Maintenance of animal

Randomly breed male Sprague Dawley (SD) rats with body weight ranges from 200 to 300gm were used in this study. The animals were purchased from M/s. Vivo Bio Tech, Hyderabad, India. Animals were randomly divided into nine groups based on their body weights consist of 6 animals of each group. They were kept individually in sterilized polypropylene cages with stainless steel top grill having provision for holding pellet feed and drinking water bottle fitted with stainless steel sipper tube. The animals were maintained as per standard protocol throughout the experiment.

Consciousness energy healing strategies

Each ingredient of the test formulation was divided into two parts. One part of the test compound was not received any sort of treatment and were defined as the untreated or control sample. The second part of the test formulation was treated with the Biofield Energy Treatment/Blessing by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi under laboratory conditions for ~3 minutes. Besides, three group of animals also received Biofield Energy Healing Treatment by Mr. Trivedi under similar laboratory conditions for ~3 minutes. The Blessing/Treatment was given to the test items by his physical presence without touching in the laboratory of Dabur Research Foundation, near New Delhi, India. After that, the Biofield Energy Treated samples was kept in the similar sealed condition and used as per the study plan. In the same manner, the control test formulation group was subjected to “sham” healer for ~3 minutes energy treatment, under the same laboratory conditions. The “sham” healer did not have any knowledge about the Biofield Energy Treatment. The Biofield Energy Treated/Blessed animals were also taken back to experimental room for further proceedings.

Experimental procedure

Seven days after acclimatization, animals were randomized and grouped based on the body weight. Dosing for groups G7 and G8 were initiated on day -15 and continued till end of the experiment. However, G1 to G6 and G9 groups were dosed from day 1 till the end of experiment. All the animals except G1 group received Vitamin D3 Deficient Diet (VDD) daily to the end of the experiment. Three weeks after the initiation of induction of VDD, all the groups were dose with the respective formulations.