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Modern Applications in Pharmacy & Pharmacology

Determination of Sensitivity Pattern of DNA Repair Genes to Anticancer Drugs

  • Open or Close Abdullahi Minjibir A1 and Muhammad Ali2*

    1Department of Pharmaceutical Technology, Kano School of Technology, Nigeria

    2Microbiology department, Kano University of Science and Technology, Nigeria

    *Corresponding author: Muhammad Ali, Department of Microbiology, Kano University of Science and Technology Wudil, Nigeria

Submission: February 12, 2018; Published: February 26, 2018

DOI: 10.31031/MAPP.2018.01.000519

ISSN 2637-7756
Volume1 Issue4


Cancer chemotherapeutic agents are known to interact with DNA and damage it in several ways. The study was aimed to determine the sensitivity pattern of DNA repair gene to two anticancer drugs namely Bleomycin and Spermidine. The haploid strain BY4741 of Saccharomyces cerevisiae and null derivatives of Ung, apnl, apn2, adk adkl, imp2’, pho8o, pho8l, pho85 were obtained from Euro scarf Research centre United Kingdom and used for the study. The mutant cells were inoculated on the solid Yeast Peptone Dextrose (YPD) agar media treated with 60μl of the agents and 8ml of 20mg/ ml of antibacterial agent, then incubated at 30 °C for 48 hours. The finding of the study showed that the parent strain (wt) is resistant to both agents by showing similar cells growth to that of control except in few mutants, suggesting that damage cause by the agents is fully repaired. The result of this research proposed that Bleomycin and Spermidine can be use as anticancer agent as its damage to cell cannot be repaired by most DNA repair genes. It’s also indicates that SPD is more potent than BLM, as some genes that can repair BLM damage but cannot repair SPD damage.

Keywords: Bleomycin; Cancer; DNA; Mutant; Spermidine

Abbreviations: DNA: Deoxyribose Nucleic Acid; SPD: Spermidine; BLM: Bleomycin; YDP: Yeast Peptone Dextrose; RNA: Ribose Nucleic Acid

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