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Modern Applications in Pharmacy & Pharmacology

Stable Labeled Isotopes as Internal Standards: A Critical Review

  • Open or Close Nageswara Rao Reddy*

    Bioanlytical/Analytical Contract Research Organization Company, USA

    *Corresponding author: Nageswara Rao Reddy, Bioanlytical/Analytical Contract Research Organization Company, USA

Submission: October 27, 2017; Published: December 18, 2017

DOI: 10.31031/MAPP.2017.01.000508

ISSN 2637-7756
Volume1 Issue2


Over the past few years, stable labeled isotopes (SILs) have played a critical role in bio-analysis, nearly replacing the use of structural analogues as internal standards. SILs are now the first choice of researchers/chemists when selecting an internal standard for day-to-day analysis to avoid process and analytical variation. However, although SILs are widely used in analytical labs today, they have challenging issues, such as the matrix effect, recovery, and ionization problems. The purpose of this review is to brief about both advantages and disadvantages of stable label internal standards from recent publications. Despite of having SILs in analytical methodology chemists observed the method issues and these were resolved later by replacing with structure analogs in their methodology.

Keywords: Liquid chromatography mass spectrometry (LC-MS/MS); Electrospray ionization (ESI); Atmospheric chemical ionization (APCI); Matrix effect and recovery

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