Abstract

In the present case study, here we report on a rape and murder of 7-year-old girl. We received partially burnt bone for human identification and biological traces of semen on the undergarment of a victim. Total DNA was extracted from bone via organic extraction/automate express and seminal stain using a differential extraction procedure. DNA was quantified through real-time PCR. DNA typing was performed using AmpFISTR® Identifiler® Plus Kit of 15 autosomal STRs loci and Amelogenin locus. 17 Y-STRs loci were analyzed by AmpFISTR® Yfiler™ Kit. Initially identification of deceased girl was confirmed by comparing the STR pattern of the deceased with that of admitted parents. Autosomal and Y-STR DNA profiles obtained from the semen stains of undergarments exactly matched with the suspect of this heinous crime. This case study report once again reiterates the significance of combined autosomal/Y STR analysis in sexual assault cases.

Keywords: Amelogenin; DNA typing; STR pattern; Y-STR